| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.24[luc2P/minP] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4411 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 用不稳定荧光素酶测定转录反应元件活性的最小启动子载体。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pGL4.24[luc2PminP]载体图谱](/uploads/载体图谱/pGL4.24[luc2PminP]载体图谱.png)
载体简介
载体序列
LOCUS pGL4.24[luc2P minP] 4411 bp ds-DNA circular SYN 29-JUN-2013
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using destabilized luciferase.
ACCESSION DQ904456
VERSION .
KEYWORDS pGL4.24[luc2P minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4411)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4411
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 141..1790
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 1794..1913
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 1965..2086
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2505..3093)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3293..4153)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4258..4306
/note="synthetic polyadenylation signal"
misc_feature 4320..4411
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
1801 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
1861 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
1921 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
1981 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
2041 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
2101 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
2161 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
2221 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
2281 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
2341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
2881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
2941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3241 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
3301 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
3361 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
3421 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
3481 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
3541 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
3601 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
3661 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
3721 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
3781 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
3841 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
3901 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
3961 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
4021 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
4081 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
4141 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
4201 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
4261 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
4321 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
4381 agtgcaagtg caggtgccag aacatttctc t
//
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